PESQUISA VETERINÁRIA BRASILEIRA

Abstract in English:

ABSTRACT.- Cazola D.O., Jorge K.S.G., Zumárraga M.J., Souza-Filho A.F., Araújo F.R. & Osório A.L.A.R. 2015. [Identification and genotyping of Mycobacterium bovis from positive cattle in skin test for tuberculosis in the State of Mato Grosso do Sul, Brazil.] Identificação e genotipagem de Mycobacterium bovis em bovinos positivos no teste intradérmico para tuberculose em Mato Grosso do Sul. Pesquisa Veterinária Brasileira 35(2):141-147. Laboratório de Micobacteriologia e Biologia Molecular, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Av. Senador Filinto Müller 2443, Campo Grande, MS 79074-460, Brazil. E-mail: danicazola@gmail.com Spoligotyping was performed in the present study to genotype Mycobacterium bovis isolates obtained from tissues of cattle that were positive in the comparative intradermal tuberculin test (CITT) in the state of Mato Grosso do Sul (Brazil). Tissue samples from 13 positive cattle from different municipalities of the state were cultured using a Stonebrink medium. The resulting colonies were subjected to Ziehl-Neelsen staining and all isolates exhibited the staining characteristics of AFB. The 13 isolates of AFB were identified by means of a multiplex PCR (mPCR) assay. The hsp65 gene was targeted for the identification of Mycobacterium spp., whereas the IS6110 insertion sequence was targeted for the identification of the Mycobacterium tuberculosis complex (MTC) and the rvd1rv2031c region was explored for the detection of Mycobacterium bovis. The spoligotyping assay was performed to genotype mycobacterial isolates. Of the 13 cattle, seven had at least one lesion suggestive of tuberculosis in the retropharyngeal, parotid and lung lymph nodes or lung. The remaining six exhibited no lesions suggestive of the disease. In the mPCR, 11 of the 13 isolates (84.6%) were positive for Mycobacterium spp., 8/13 (61.5%) were positive for the MTC and 7/13 (53.8%) were positive for M. bovis. Based on the spoligotyping, eight isolates were grouped into three different groups of genotypes and one isolate exhibited an orphan type. Four isolates exhibited spoligotype pattern SB0121, while two isolates were associated with the pattern SB1145, another two were associated with pattern SB0881 and one was associated with pattern SB0140. Spoligotyping confirmed the genetic diversity present among isolates found in the state of Mato Grosso do Sul. In addition, SB0121 was confirmed as the predominant profile.

• Mycobacterium bovis tuberculosis tuberculose bovina

Abstract in Portuguese:

RESUMO.- Cazola D.O., Jorge K.S.G., Zumárraga M.J., Souza-Filho A.F., Araújo F.R. & Osório A.L.A.R. 2015. [Identification and genotyping of Mycobacterium bovis from positive cattle in skin test for tuberculosis in the State of Mato Grosso do Sul, Brazil.] Identificação e genotipagem de Mycobacterium bovis em bovinos positivos no teste intradérmico para tuberculose em Mato Grosso do Sul. Pesquisa Veterinária Brasileira 35(2):141-147. Laboratório de Micobacteriologia e Biologia Molecular, Faculdade de Medicina Veterinária e Zootecnia, Universidade Federal de Mato Grosso do Sul, Av. Senador Filinto Müller 2443, Campo Grande, MS 79074-460, Brazil. E-mail: danicazola@gmail.com Neste estudo, realizou-se genotipagem de isolados de Mycobacterium bovis, provenientes de amostras de tecidos de bovinos positivos no teste cervical comparativo (TCC) para tuberculose em Mato Grosso do Sul, por meio da técnica de spoligotyping. Tecidos de 13 bovinos positivos, oriundos de diferentes municípios do estado, foram cultivados em meio de Stonebrink. As colônias resultantes foram submetidas à coloração de Ziehl-Neelsen e todos os isolados apresentaram características tintoriais de BAAR. Os 13 isolados de BAAR foram identificados por PCR multiplex (mPCR). O gene hsp65 foi alvo para identificação de Mycobacterium spp, a sequência de inserção IS6110 foi alvo para identificação de complexo Mycobacterium tuberculosis (CMT) e a região rvd1rv2031c foi explorada para detecção de M. bovis. Os isolados micobacterianos foram genotipados pela técnica de spoligotyping. Dos 13 bovinos, sete tinham pelo menos uma lesão sugestiva de tuberculose em linfonodos retrofaríngeos, parotídeos e pulmonares ou no pulmão, e em seis não foram encontradas lesões visíveis sugestivas da doença. Na mPCR, 11/13 (84,6%) isolados foram positivos para Mycobacterium spp; 8/13 (61,5%) positivos para CMT e 7/13 (53,8%) positivos para M. bovis. Com base no spoligotyping, oito isolados de BAAR foram agrupados dentro de três diferentes agrupamentos de genótipos e uma amostra remanescente apresentou perfil único, sendo quatro isolados com padrão de espoligotipo SB0121, dois SB1145, dois SB0881 e um SB0140. A técnica de spoligotyping demonstrou que há diversidade genética entre os espoligotipos presentes no estado de Mato Grosso do Sul, embora predomine o perfil SB0121.

Abstract in English:

ABSTRACT.- Melo E.S.P., Souza I.I.F., Ramos C.A.N., Osório A.L.A.R., Nascimento V.A. & Araújo F.R. 2014. [Skin test with recombinant protein of Mycobacterium bovis as antigen in Cavia porcellus.] Teste intradérmico com proteínas recombinantes de Mycobacterium bovis como antígenos em Cavia porcellus. Pesquisa Veterinária Brasileira 34(10):957-962. Programa de Pós-Graduação em Ciência Animal, Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso do Sul, Av. Senador Filinto Mülller 2443, Cidade Universitária, Campo Grande, MS 79070-900, Brazil. E-mail: elainespmelo@hotmail.com The intradermal skin test for diagnosis of bovine tuberculosis has been used the purified protein derivative (PPD) of Mycobacterium bovis, that is able to induce a hypersensitivity reaction in infected animals. However, shows low specificity due to the occurrence of cross reactions with other mycobacteria. Thus, the aim of this study was to produce recombinant proteins (ESAT-6, PE13, PE5 and ESX-1) of Mycobacterium bovis and assess them as antigens in skin test using guinea pigs (Cavia porcellus) as a model, and check if the conditions employed in the purification (native or denaturing condition) interfere in the antigenic performance of these proteins. The proteins were tested in guinea pigs previously sensitized with inactivated M. bovis strain AN5, individually (160 µg/µl), or as a mixed cocktail (40 µg each). The cocktail of proteins induced hypersensitivity reactions in sensitized animals significantly (p=0.002) higher than those observed in non-sensitized animals, allowing differentiation. On the other hand, the proteins individually were not able to promote this differentiation. The conditions of solubilization and purification influenced the antigenic performance of the protein ESAT-6, since, when produced in denaturing condition triggered nonspecific reaction in non-sensitized animals. Whereas when produced under native conditions and used at concentrations (6, 12, 24 and 48µg/µl) induced a significant response only in sensitized animals, confirming its potential as antigen.

• Tuberculosis Mycobacterium bovis tuberculose

Abstract in Portuguese:

RESUMO.- Melo E.S.P., Souza I.I.F., Ramos C.A.N., Osório A.L.A.R., Nascimento V.A. & Araújo F.R. 2014. [Skin test with recombinant protein of Mycobacterium bovis as antigen in Cavia porcellus.] Teste intradérmico com proteínas recombinantes de Mycobacterium bovis como antígenos em Cavia porcellus. Pesquisa Veterinária Brasileira 34(10):957-962. Programa de Pós-Graduação em Ciência Animal, Faculdade de Medicina Veterinária, Universidade Federal de Mato Grosso do Sul, Av. Senador Filinto Mülller 2443, Cidade Universitária, Campo Grande, MS 79070-900, Brazil. E-mail: elainespmelo@hotmail.com O teste intradérmico para o diagnóstico da tuberculose bovina utiliza derivados proteicos purificados (PPD) de Mycobacterium bovis que são capazes de induzir reações de hipersensibilidade em animais infectados. No entanto, apresenta baixa especificidade devido à ocorrência de reações cruzadas com outras micobactérias. Neste sentido, o objetivo desse trabalho foi produzir proteínas recombinantes (ESAT-6, PE13, PE5 e ESX-1) de Mycobacterium bovis e avaliá-las como antígenos em teste intradérmico utilizando Cavia porcellus como modelo, e verificar se as condições empregadas na purificação (nativa ou desnaturante) interferem no desempenho antigênico dessas proteínas. As proteínas foram testadas em Cavia porcellus previamente sensibilizados com cepa M. bovis AN5 inativada, individualmente (160 µg) ou combinadas na forma de um coquetel (40 µg cada). O coquetel de proteínas induziu reações de hipersensibilidade nos animais sensibilizados significativamente superiores (p=0,002) as observadas nos animais não sensibilizados, possibilitando diferenciação. No entanto, as proteínas isoladamente não foram capazes de promover essa diferenciação. As condições de solubilização e purificação influenciaram o desempenho antigênico da proteína ESAT-6, pois, quando produzida em condição desnaturante desencadeou reações inespecíficas nos animais não sensibilizados, enquanto que aquela produzida em condições nativas e aplicada em concentrações de 6, 12, 24 e 48µg induziu reações significativas apenas nos animais sensibilizados, confirmando o seu potencial como antígeno.

Abstract in English:

ABSTRACT.- Souza F.G., Osório A.L.A.R., Csordas B.G., Prado R.Q., Elisei C., Soares C.O., Araújo F.R., Fragoso S.P. & Rosinha G.M.S. 2009. [Development and evaluation of a strain of Brucella abortus gotten by the knockout of the virB10 gene.] Desenvolvimento e avaliação de uma cepa knockout de Brucella abortus obtida pela deleção do gene virB10. Pesquisa Veterinária Brasileira 29(11):943-950. Programa de Pós-Graduação, Mestrado em Ciência Animal, Faculdade de Medicina Veterinária e Zootecnia da Universidade Federal de Mato Grosso do Sul, Campo Grande, MS 79070-900, Brazil. E-mail: fabicientista@gmail.com Brucella spp. are intracellular facultative gram-negative bacteria which are pathogenic for many species of mammals, causing brucellosis, a worldwide spread zoonosis. Therefore the search for more efficient alternatives of control, as the development of new potential immunogens is necessary. In this study, we knockouted virB10 from Brucella abortus S2308 strain, generating a mutant strain probably incapable to produce the corresponding native protein. The gene virB10 is part of an operon that codifies for type IV secretion system, which is essential for the intracellular survival and multiplication of the bacteria in host cells. The knockout was carried through by the construction of the suicidal plasmid pBlue: virB10: kan and eletroporation in eletrocompetent cells of B. abortus S2308, leading to the exchange of the wild gene for the interrupted gene, containing the gene of resistance to kanamycin, for double homologous recombination. BALB/c mice were inoculated with S19, RB-51, DvirB10 strains of B. abortus and S2308 wild strain; the results demonstrated that the BALB/c mice inoculated with S19 and BALB/c mice inoculated with S2308 presented faster fall of trend line, when compared with the too much groups, for bacterial recovery (BR) and esplenic weight (EW) respectively. The groups that received DvirB10 S2308 B. abortus and RB-51 demonstrated similar behavior for both the characteristics. In the sixth week postinoculation, the results for BR (log UFC ± standart deviations) and EW (esplenic weight ± standart deviations), respectively, showed: groups inoculated with strains S2308 (4,44±1,97 and 0,44±0,11), S19 (1,83±2,54 and 0,31±0,04), RB-51 (0,00±0,00 and 0,20±0,01) and DvirB10 S2308 (1,43±1,25 and 0,19±0,03). Considered the bacterial clearance, all the groups differed statistical from the group that received S2308 (p<0,0001), the group inoculated with DvirB10 S2308 B. abortus was similar to the S19 group (p=0,4302) and different of group RB-51 (p=0,0063). The evaluation of the persistence of the strains showed that virB10 is essential for the maintenance of the virulence. These results support other studies concerning the immunogenic potential of this mutant strain.

• Brucella abortus

Abstract in Portuguese:

RESUMO.- Souza F.G., Osório A.L.A.R., Csordas B.G., Prado R.Q., Elisei C., Soares C.O., Araújo F.R., Fragoso S.P. & Rosinha G.M.S. 2009. [Development and evaluation of a strain of Brucella abortus gotten by the knockout of the virB10 gene.] Desenvolvimento e avaliação de uma cepa knockout de Brucella abortus obtida pela deleção do gene virB10. Pesquisa Veterinária Brasileira 29(11):943-950. Programa de Pós-Graduação, Mestrado em Ciência Animal, Faculdade de Medicina Veterinária e Zootecnia da Universidade Federal de Mato Grosso do Sul, Campo Grande, MS 79070-900, Brazil. E-mail: fabicientista@gmail.com Brucella spp. são bactérias gram-negativas, intracelulares facultativas que são patogênicas para muitas espécies de mamíferos causando a brucelose, uma zoonose difundida mundialmente. Por isso a busca de alternativas de controle mais eficientes se faz necessário como o desenvolvimento de novas cepas que possam ser testadas como potenciais imunógenos. Neste estudo realizou-se a deleção do gene virB10 da cepa S2308 de Brucella abortus gerando uma cepa knockout provavelmente incapaz de produzir a proteína nativa correspondente. O gene virB10 faz parte de um operon que codifica para um sistema de secreção do tipo IV, essencial para a sobrevivência intracelular e multiplicação da bactéria em células hospedeiras. A deleção foi realizada pela construção do plasmídeo suicida pBlue:virB10:kan e eletroporação deste em células eletrocompetentes de B. abortus S2308, ocorrendo a troca do gene selvagem pelo gene interrompido, com o gene de resistência a canamicina, por recombinação homóloga dupla. Camundongos BALB/c foram inoculados com as cepas S19, RB-51, DvirB10 de B. abortus e B. abortus S2308 selvagem; os resultados demonstraram que camundongos BALB/c inoculados com S19 e camundongos BALB/c inoculados com S2308 apresentaram queda mais rápida de linha de tendência, quando comparadas aos demais grupos, para recuperação bacteriana (RB) e peso esplênico (PE) respectivamente. Os grupos que receberam DvirB10 S2308 de B. abortus e RB-51 demonstraram comportamento semelhante para ambas as características. Na sexta semana após a inoculação, os resultados para RB (log de UFC ± desvio padrão) e PE (peso esplênico ± desvio padrão), respectivamente, mostraram: grupos inoculados com as cepas S2308 (4,44±1,97 e 0,44±0,11), S19 (1,83±2,54 e 0,31±0,04), RB-51 (0,00±0,00 e 0,20±0,01) e DvirB10 S2308 (1,43±1,25 e 0,19±0,03). Considerado o clearance bacteriano, todos os grupos diferiram estatisticamente do grupo que recebeu S2308 (p<0,0001), o grupo inoculado com DvirB10 S2308 de B. abortus foi semelhante ao grupo S19 (p=0,4302) e diferente do grupo RB-51 (p=0,0063). A avaliação da persistência revelou que o gene virB10 é essencial para a manutenção da virulência da bactéria. Os resultados obtidos possibilitarão que outras pesquisas sejam realizadas avaliando o potencial imunogênico desta cepa mutante.

Abstract in English:

ABSTRACT.- Figueiredo A.O., Pellegrin A.O., Gonçalves V.S.P., Freitas E.B., Monteiro L.A.R.C., Oliveira J.M. & Osório A.L.A.R. 2009. [Prevalence and risk factors for bovine leptospirosis in Mato Grosso do Sul, Brazil.] Prevalência e fatores de risco para a leptospirose em bovinos do Mato Grosso do Sul. Pesquisa Veterinária Brasileira 29(5):375-381. Programa Mestrado em Ciência Animal, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS 79070-900, Brazil. E-mail: line_figueiredo@yahoo.com.br The prevalence of anti-Leptospira spp. antibodies was estimated for female cattle aged 24 months or older. The sample comprised 178 herds from 22 counties in the state of Mato Grosso do Sul, Brazil. The risk factors associated with the presence of infeccion were investigated. A total of 2,573 blood serum samples were tested against 10 leptospira serovars using the microagglutination test (MAT). Titers of 100 or higher for one or more serovars were detected in 1,801 females (98.8%) from 161 herds (96.5%). Serovar Hardjo (65.6%) was the most frequent, followed by serovar Wolffi (12.3%). These results suggest that bovine leptospirosis is widespread in all the counties under study, with a high prevalence both at the animal and the herd level. Beef farms and the Zebu breed were associated to the higher risk of herd infection by leptospiras.

• Leptospirosis Leptospirose

Abstract in Portuguese:

ABSTRACT.- Figueiredo A.O., Pellegrin A.O., Gonçalves V.S.P., Freitas E.B., Monteiro L.A.R.C., Oliveira J.M. & Osório A.L.A.R. 2009. [Prevalence and risk factors for bovine leptospirosis in Mato Grosso do Sul, Brazil.] Prevalência e fatores de risco para a leptospirose em bovinos do Mato Grosso do Sul. Pesquisa Veterinária Brasileira 29(5):375-381. Programa Mestrado em Ciência Animal, Universidade Federal de Mato Grosso do Sul, Campo Grande, MS 79070-900, Brazil. E-mail: line_figueiredo@yahoo.com.br The prevalence of anti-Leptospira spp. antibodies was estimated for female cattle aged 24 months or older. The sample comprised 178 herds from 22 counties in the state of Mato Grosso do Sul, Brazil. The risk factors associated with the presence of infeccion were investigated. A total of 2,573 blood serum samples were tested against 10 leptospira serovars using the microagglutination test (MAT). Titers of 100 or higher for one or more serovars were detected in 1,801 females (98.8%) from 161 herds (96.5%). Serovar Hardjo (65.6%) was the most frequent, followed by serovar Wolffi (12.3%). These results suggest that bovine leptospirosis is widespread in all the counties under study, with a high prevalence both at the animal and the herd level. Beef farms and the Zebu breed were associated to the higher risk of herd infection by leptospiras.

Abstract in English:

ABSTRACT.- Almeida R.F.C., Madruga C.R., Soares C.O., Fernandes M.C., Carvalho N.M., Jorge K.S.G. & Osório A.L.A.R. 2006. [Specific immune response of cattle to experimental sensibilization by inactivated Mycobacterium bovis and Mycobacterium avium.] Resposta imune específica de bovinos experimentalmente sensibilizados com inóculos inativados de Mycobacterium bovis e Mycobacterium avium. Pesquisa Veterinária Brasileira 26(4):195-200. Departamento de Medicina Veterinária da Universidade Federal de Mato Grosso do Sul, Cidade Universitária, Campo Grande, MS 79070-900, Brazil. E-mail: analudmv@nin.ufms.br The presumptive diagnosis of bovine tuberculosis is based on analysis of the immune response to micobacterial antigens. This experimental simulation of sensibilization by Mycobacterium bovis and Mycobacterium avium in cattle aimed to verify the immune response by both the cervical comparative test and the evolution of the specific production of gamma-interferon, and also to identify interference of unspecified reactions by M. avium on the test results. The results support that the experimental animals started a response of delayed hypersensitivity to the inactivated bacilli, and that both diagnostic tests for bovine tuberculosis were efficient for the identification of animals sensitized with M. bovis and for discrimination of reactions generated by inoculation of cattle with M. avium.

• Mycobacterium bovis Mycobacterium avium gamma-interferon tuberculin immune response

Abstract in Portuguese:

ABSTRACT.- Almeida R.F.C., Madruga C.R., Soares C.O., Fernandes M.C., Carvalho N.M., Jorge K.S.G. & Osório A.L.A.R. 2006. [Specific immune response of cattle to experimental sensibilization by inactivated Mycobacterium bovis and Mycobacterium avium.] Resposta imune específica de bovinos experimentalmente sensibilizados com inóculos inativados de Mycobacterium bovis e Mycobacterium avium. Pesquisa Veterinária Brasileira 26(4):195-200. Departamento de Medicina Veterinária da Universidade Federal de Mato Grosso do Sul, Cidade Universitária, Campo Grande, MS 79070-900, Brazil. E-mail: analudmv@nin.ufms.br The presumptive diagnosis of bovine tuberculosis is based on analysis of the immune response to micobacterial antigens. This experimental simulation of sensibilization by Mycobacterium bovis and Mycobacterium avium in cattle aimed to verify the immune response by both the cervical comparative test and the evolution of the specific production of gamma-interferon, and also to identify interference of unspecified reactions by M. avium on the test results. The results support that the experimental animals started a response of delayed hypersensitivity to the inactivated bacilli, and that both diagnostic tests for bovine tuberculosis were efficient for the identification of animals sensitized with M. bovis and for discrimination of reactions generated by inoculation of cattle with M. avium.

Abstract in English:

ABSTRACT.- Monteiro L.A.R.C., Pellegrin A.O., Ishikawa M.M. & Osório A.L.A.R. 2006. [Epidemiological investigation of bovine brucellosis in an extract of Mato Grosso do Sul, Brazil.] Investigação epidemiológica da brucelose bovina em um estrato do Estado de Mato Grosso do Sul. Pesquisa Veterinária Brasileira 26(4):217-222. Programa Mestrado em Ciência Animal, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS 79070-900, Brazil. E-mail: leticiacmonteiro@yahoo.com.br The study aimed to estimate the prevalence of bovine brucellosis in 22 counties which make up the region Extract 1 of the State of Mato Grosso do Sul, Brazil, in order to identify risk factors associated with the infection. The sample region encompasses an area of 70,214.1 km² and represents 19.7% of the State. The region studied has about 5.7 million head of cattle, corresponding to 23% of the total of 24.9 million cattle in the State of Mato Grosso do Sul. On 210 farms, between December 2003 and March 2004, 2,376 blood samples were collected from cows, aged 24 months or older, for serial diagnostic tests. Screening through the buffered acidified antigen test was confirmed by the 2-mercaptoetanol test. On the occasion of sample collection a questionnaire with information related to identification, kind of cattle and management practices was filled out. In individual animals the real prevalence was estimated at 5.6%, and in the cattle herds at 37.3%. The variables, which presented association through odds ratio (OR), univariate analysis and 95% confidence interval (CI) with serum positivity for brucellosis, were: the exploration of beef cattle (OR = 2.82, 95% CI = 1.49-5.34), Zebu breed (OR = 2.62, 95% CI = 1.40-4.88) and abortion (OR = 1.83, 95% CI = 1.01-3.33). The results shown here demonstrate, despite the prevalence of brucellosis in the extract of Mato Grosso do Sul studied, that the control of the disease may depend on adoption of a program focusing upon the exploration of beef cattle, the Zebu breed and the occurrence of abortion.

• Brucellosis cattle epidemiology seroprevalence

Abstract in Portuguese:

ABSTRACT.- Monteiro L.A.R.C., Pellegrin A.O., Ishikawa M.M. & Osório A.L.A.R. 2006. [Epidemiological investigation of bovine brucellosis in an extract of Mato Grosso do Sul, Brazil.] Investigação epidemiológica da brucelose bovina em um estrato do Estado de Mato Grosso do Sul. Pesquisa Veterinária Brasileira 26(4):217-222. Programa Mestrado em Ciência Animal, Universidade Federal do Mato Grosso do Sul, Campo Grande, MS 79070-900, Brazil. E-mail: leticiacmonteiro@yahoo.com.br The study aimed to estimate the prevalence of bovine brucellosis in 22 counties which make up the region Extract 1 of the State of Mato Grosso do Sul, Brazil, in order to identify risk factors associated with the infection. The sample region encompasses an area of 70,214.1 km² and represents 19.7% of the State. The region studied has about 5.7 million head of cattle, corresponding to 23% of the total of 24.9 million cattle in the State of Mato Grosso do Sul. On 210 farms, between December 2003 and March 2004, 2,376 blood samples were collected from cows, aged 24 months or older, for serial diagnostic tests. Screening through the buffered acidified antigen test was confirmed by the 2-mercaptoetanol test. On the occasion of sample collection a questionnaire with information related to identification, kind of cattle and management practices was filled out. In individual animals the real prevalence was estimated at 5.6%, and in the cattle herds at 37.3%. The variables, which presented association through odds ratio (OR), univariate analysis and 95% confidence interval (CI) with serum positivity for brucellosis, were: the exploration of beef cattle (OR = 2.82, 95% CI = 1.49-5.34), Zebu breed (OR = 2.62, 95% CI = 1.40-4.88) and abortion (OR = 1.83, 95% CI = 1.01-3.33). The results shown here demonstrate, despite the prevalence of brucellosis in the extract of Mato Grosso do Sul studied, that the control of the disease may depend on adoption of a program focusing upon the exploration of beef cattle, the Zebu breed and the occurrence of abortion.

Abstract in English:

ABSTRACT.- Salvador S.C., Lemos R.A.A., Riet-Correa F., Roehe P.M. & Osório A.L.A.R. 1998. [Meningoencephalitis in cattle caused by bovine herpesvirus-5 in Mato Grosso do Sul and São Paulo.] Meningoencefalite em bovinos causada por herpesvírus bovino-5 no Mato Grosso do Sul e São Paulo. Pesquisa Veterinária Brasileira 18(2):76-83. Depto Medicina Veterinária, Universidade Federal do Mato Grosso do Sul, Cx. Postal 649, Campo Grande, MS 79070-900, Brazil. Fifteen outbreaks of bovine herpesvirus-type 5 (BHV-5) infection were diagnosed from August 1993 to December 1996. Fourteen outbreaks occurred in the State of Mato Grosso do Sul and one in the State of São Paulo. Cattle 6 to 60 months old were affected. Morbidity reached 0.05% to 5% and case fatality rate was nearly 100%. The disease occurred in diferente municipalities and at different times of the yea1: Clinical signs were exclusively nervous, and the clinical course varied from 1 to 15 days. The main histologic lesions were meningitis, diffuse encephalitis and necrosis of the cerebral cortex with intranuclear inclusion bodies in astrocytes and neurons. BHV-5 was isolated from 11 out of 12 brains of infected animals inoculated in calf testis cells and MDBK cells. The vírus was identified by immunoperoxidase stainingwith use of monoclonal specific antibodies. Outbreaks of infection by BHV-5 represent 5% of the total number of bovine cases submitted for diagnosis to the Clinical Hospital of the University of Mato Grosso do Sul. These results indicate the importance of the disease in Mato Grosso do Sul and the need for its differentiation from other diseases which affect the nervous system.

• Meningoencephalitis bovine herpesvirus-5 BHV-5 cortical cerebral necrosis cattle Mato Grosso do Sul Meningoencefalite herpesvírus bovino-5 polioencefalomalacia bovinos Mato Grosso do Sul.

Abstract in Portuguese:

RESUMO.- Salvador S.C., Lemos R.A.A., Riet-Correa F., Roehe P.M. & Osório A.L.A.R. 1998. [Meningoencephalitis in cattle caused by bovine herpesvirus-5 in Mato Grosso do Sul and São Paulo.] Meningoencefalite em bovinos causada por herpesvírus bovino-5 no Mato Grosso do Sul e São Paulo. Pesquisa Veterinária Brasileira 18(2):76-83. Depto Medicina Veterinária, Universidade Federal do Mato Grosso do Sul, Cx. Postal 649, Campo Grande, MS 79070-900, Brazil. Quinze focos de meningoencefalite por herpesvírus bovino-5 (BHV-5) foram diagnosticados entre agosto de 1993 e dezembro de 1996, sendo 14 provenientes do estado do Mato Grosso do Sul e um do estado de São Paulo. A doença ocorreu em diversos municípios e em diferentes épocas do ano. Foram afetados bovinos de 6 a 60 meses de idade, com uma morbidade de 0,05% a 5% e letalidade próxima a 100%. Os sinais clínicos foram exclusivamente nervosos e o curso da enfermidade variou de 1 a 15 dias. As principais lesões histológicas detectadas foram meningite e encefalite difusa com malacia do córtex cerebral e presença de corpúsculos de inclusão intranucleares em astrócitos e neurônios. O vírus foi isolado do cérebro de 11 de um total de 12 animais, e sua identidade confirmada por imunoperoxidase, utilizando-se anticorpos monoclonais específicos. Os surtos de encefalite por BHV-5 representam 5% dos diagnósticos realizados em bovinos pelo Hospital Veterinário da Universidade Federal do Mato Grosso do Sul. Os resultados deste trabalho evidenciam a importância da doença no Mato Grosso do Sul e indicam a necessidade de incluir a encefalite por BHV-5 no diagnóstico diferencial de outras doenças do sistema nervoso de bovinos frequentes no Estado.